Blog

Nancy Sajjadi

Developments in Bioassays

For over 30 years, I have been involved in the quality control of experimental biologics and have witnessed both incredible outcomes and tragic disappointments in addressing many different unmet clinical needs. I recently attended a conference…

Anti-vector immunoglobulin induced by retroviral vectors

This paper summarizes work to character vector immunogenicity. Studies showed that intramuscular (i.m.) administration of retroviral vector induces strong anti-vector Ig responses, in a dose-dependent fashion. Although such anti-vector Ig was…

Evidence for localization of biologically active recombinant retroviral vector to lymph nodes in mice injected intramuscularly.

Results of these studies indicated that the localization of retroviral DNA to the injection site and regional lymph nodes may play a role in the induction of the product directed, specific CTL responses detected in splenocyte populations isolated…

Recombinant retroviral vector interferes with the detection of amphotropic replication competent retrovirus in standard culture assays

This paper has been cited over 40 times.  It summarizes studies that revealed that recombinant retroviral vector can interfere with the detection of low-level RCR in culture and that such interference can be partially overcome by culturing…

Recombinant retroviral vector delivered intramuscularly localizes to the site of injection in mice

Two of the main safety considerations associated with the direct administration of recombinant retrovirus are the generation of replication-competent retrovirus in vector-producing cell lines and inadvertent germ-line transduction. In this early…